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Author Topic: Breeding Journal, Species: Chromis viridis (Green Chromis Damselfish)  (Read 12309 times)
mpedersen
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« on: August 23, 2010, 12:19:01 pm »

Breeding Journal DataSheet


General

Species: Chromis viridis, aka. Green Chromis
Social Structure: Beeing kept in a trio (1m/2f)
Size of Individuals: Male 3" easily, females 2.25 to 2.5" perhaps
Age of Individuals: unknown, wild caught
Date added to Tank: 8/22/2010, from Jay Hansen, as a spawning trio.

Broodstock Tank Details
Size of Tank: 29 gallon
Substrate Details: none at this time
Filtration Details: small hang on the back power filter (Aquaclear I think)
Water Changes: Will probably be 5 gallons weekly
Water Temperature: 80-81F
Lighting: Single stock strip light
Lighting Cycle: manual, probably 12-14 hours per day
Other Tank Inhabitants: none at this time

Broodstock Feeding Details
Food Types: They will be offered everything I have, be it Mysis, Brine Shrimp, Frozen Mixed foods, Fish Eggs, Pellets, Flakes etc
Feeding Schedule: They will be fed probably 3 times per day

Spawning Details
Date of First Spawn:9-7-2010
Spawn Time of Day:unknown
Dates of Consecutive Spawns: 9-8-2010
Courtship Details: Not sure, but the male selects the nest site and it appears that he darkens in color.  He displays to the females, presumably to lure receptive females to the nest site.  Jay, the prior owner of the pair, suggests that there is a roughly weekly spawning routine.
Egg Size: 600 microns X 500 microns
Egg Color: clear
Egg Count: thousands

Hatch Details
Hatch Date:
Hatch Time of Day:
# Days after Spawn:
Larvae Description:


Larval Tank Details
Temperature:
Size of Larval Tank:
Substrate Details:
Other Tank Decor:
Filtration Details:
Lighting:
Lighting Cycle:
Water Changes:

Larval Feeding Details
Food Types:
Feeding Schedule:

Metamorphosis/Settlement
Date of Settlement Start:
Days after Hatch:
Date of Settlement End:
Description of Fry:

Grow-Out Tank Details
Temperature:
Size of Grow-Out Tank:
Substrate Details:
Other Tank Decor:
Filtration Details:
Lighting:
Lighting Cycle:
Water Changes:
Size at Transfer:
Age at Transfer:

Grow-Out Feeding Details
Food Types:
Feeding Schedule:

Additional Information
Miscellaneous Information:

I started this thread today because Jay gave me his spawning trio.  They SPAWNED on 8-22-2010, and it already looks like the male is going to get a spawn out of another female today (8-23) - he is making swooping passes over the back glass, and his "spermapositor" (what do they actually call it?) is down as of 11:00 AM.  Yes, I feel like it's cheating a little bit, but I will make efforts to document and rear, as well as perhaps get Jay to share his own story about these fish and perhaps submit a breeding journal covering his experiences.
« Last Edit: September 09, 2010, 12:35:34 am by mpedersen » Logged

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« Reply #1 on: September 08, 2010, 10:31:16 pm »

9-8-2010 - had courtship in the AM of 9-7-2010.  Shot a video.  Noticed what I thought were eggs last night, then MORE this afternoon.  While on the phone with Jay, he confirmed based on my description that this is how they spawn.  Now, here's the crazy part, there are THOUSANDS of eggs covering MOST of a massively largely tile (I think they're 8 X 8, but may even be 12 X 12")?  I need to take a measurement.  The eggs are also VERY small...i.e. possibly smaller than the Oxymonacanthus longirostris eggs.  Videos, photos, and measurements coming, but in case I don't get to them right away...figured I needed to DOCUMENT!
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« Reply #2 on: September 08, 2010, 11:57:00 pm »

Ok, the video I shot yesterday vanished, but I have other courtship type videos from 9-5-2010!
http://www.youtube.com/watch?v=btG_PLZhgHw

Here's some pictures of the spawn(s) - the big tile in back is 8 inches X 10 inches:



The red outline shows the approximate area and size of the Chromis nest:








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« Reply #3 on: September 09, 2010, 12:42:24 am »

More tonight - pulled some eggs off the tile with a pipette.  got video and microscope shots.

First, 200 X magnification video - you can see the heartbeat.  0.6 mm X 0.5 mm egg - they are FREAKIN TINY!
http://www.youtube.com/watch?v=7Zu8hA3UD8c

Here's microscope pictures, 10X, 60X and 200X:

















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« Reply #4 on: September 09, 2010, 11:22:40 am »

9-9-2010

Somewhere between 1:00 AM and 9:30 AM we had a massive hatch.  The 29 gallon broodstock tank was simply covered in larval Green Chromis.  Got lots of pictures and videos today...

Official measurement - 2.1 MM in length at hatching.

10X Video - http://www.youtube.com/watch?v=9d01LqVNo0M
60X Video - http://www.youtube.com/watch?v=URHWL1JrQzI
200X Video - http://www.youtube.com/watch?v=HsBd3PeSupk

Photos at 10X, 60X and 200X, shot with the QX5



























More  later
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« Reply #5 on: September 09, 2010, 12:19:30 pm »

Took a moment at lunch to upload the stuff I shot with Renee's camera.

Video of the prolarvae in tank - http://www.youtube.com/watch?v=HwYv6BA_dRE

A couple shots in the tank:



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« Reply #6 on: September 09, 2010, 09:22:55 pm »

This evening I added 5 gallons worth of rotifers and 50 drops of RotiGreen paste (thawed) as well as 50 drops of ChloramX.  Basically, greenwater technique - figured I need a good amount of enriched baby rotifers IN THE TUB when first feeding starts sometime tomorrow.
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« Reply #7 on: September 09, 2010, 10:44:12 pm »

New images - maybe 13 hours after the last set.  Appear to have grown to 2.3 mm

Videos

60X - http://www.youtube.com/watch?v=v6N5iGClwdg
200X - http://www.youtube.com/watch?v=x3EZY6VXoCA

Photos

10x, 60X and 200X - calling it  0.5 days post hatch...

































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« Reply #8 on: September 10, 2010, 10:00:37 am »

9-10-2010, 1 day post hatch
Another hatch this morning which was promptly eaten by the parents when they woke up (they "sleep" at night, which allows for the babies to sit in the tank unmolested until I turn on the lights in the morning...no the filtration is a small hang on the back which doesn't turn the water over that fast...thus many still in the water column)

Looks like Debbie Berlin's (of MASM) observations are going to carry through on my own and today should be first feeding day.  As of 24 hours, they don't look ready yet.  I added 30 or 40 more drops of Rotigreen to the Black Round Tub (still running at 10 gallons of water) as it was looking a bit clear this morning already.  Babies have now grown to 2.5 - 2.6 mm.

200X Video - http://www.youtube.com/watch?v=yab1Itf9Zuc

Pictures at 10X, 60X and 200X













note rotifers in next couple images for size comparison










« Last Edit: September 10, 2010, 10:02:25 am by mpedersen » Logged

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« Reply #9 on: September 11, 2010, 09:40:32 pm »

More updates.  First, videos and images taken last night, 9-10-2010, at 1.5 days post hatch.

Video, 60X - http://www.youtube.com/watch?v=pifGtzBAO7M

Photos @ 10X and 60X













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« Reply #10 on: September 11, 2010, 09:45:05 pm »

9-11-2010

We're now at 2.5 days post hatch. Added another 60 drops of RotiGreen this AM.  Turned on a light closer to the tub as well.  It looks like perhaps maybe we have some feeding - hard to say.  Still 2.5 mm in total length.

Video at 200X
- http://www.youtube.com/watch?v=p959-VuZubw

Images at 10, 60 and 200X



















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« Reply #11 on: September 12, 2010, 11:05:19 pm »

So, 9-12-2010 - something like 50 drops of Roti Green went in this morning.  Topped off for evaporation.  This evening, the 10 gallons of water in the Black Round Tub were almost clear again, so I once again added in RotiGreen, another 50 drops, and 50 drops of ChloramX as well.  Looks like there's still many babies.  We're at 3.5 days post hatch now.  Still around 2.5, maybe 2.6 MM total length.

Videos
60X - http://www.youtube.com/watch?v=HnQJShts-Eg
200X #1 - http://www.youtube.com/watch?v=0ouEeoBz-qQ
200X #2 - http://www.youtube.com/watch?v=akZHNy63Clk

Images at 10X, 60X, and 200X





































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« Reply #12 on: September 13, 2010, 10:53:17 pm »

9-13-2010, 4.5 days post hatch now.  Die offs certainly kicking in.  Number of larvae has drastically fallen today.  Most all that I sampled looked weak or starving.  This could mean the end of the run in another day or two...

Videos

60X - http://www.youtube.com/watch?v=B20sf5LcyKg
200X - http://www.youtube.com/watch?v=8n2BYK0kQ-k

Images at 10X, 60X and 200X





















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« Reply #13 on: September 19, 2010, 11:02:08 pm »

I have pictures from 5+ days to post (need to export them from the microscope software).  The last living larvae I saw was on the morning of the 6th day.  It seemed that the babies were kind feeding for a day or two there and then stopped...that's how this run appears to have worked out.  6 days post hatch is the current milestone.  I should do a starvation trial.
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« Reply #14 on: September 21, 2010, 12:37:11 am »

OK, from 9-14, in the morning I think, thus 5 days post hatch.  This is the last I got from the first run, although I know the run made it 1 more day, as a solitary baby was observed on the morning of 9-15.  Clearly, this fish has nothing in its gut.

Video @ 200X - http://www.youtube.com/watch?v=nXcHNvzzhVA

Images at 10X, 60X and 200X.




















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